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3AE-phosphoadenosine 5AE-phosphate (PAP) a 3AE-phosphorylated nucleotide is found in almost all organisms and is obtained as a by-product of sulfur and lipid metabolism.
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In the extracellular space ecto-5AE-nucleotidase (CD73) dephosphorylates adenosine triphosphate (ATP) to produce adenosine. Adenosine has four receptors namely A1R A2AR A2BR and A3R. Adenosine plays a key role in the osteogenic differentiation. A1R induces osteoclast differentiation and A2AR induces osteoblast differentiation.
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GTP also known as Guanosine-5 -triphosphate can be utilized in various molecular biology applications such as in vitro transcription RNA amplification and siRNA synthesis Additionally GTP plays a critical regulatory role in signal transduction as a phosphate or pyrophosphate messenger it activates G-proteins which subsequently initiate protein kinase-mediated signaling cascades thus influencing cellular behaviors including cell proliferation and differentiation GTP also serves as a high-energy nucleotide precursor participating in enzymatic synthesis processes of DNA and RNA This product is prepared from GTP trisodium salt with purity 99% (HPLC) as a clear colorless aqueous solution at a concentration of 100 mM and pH (25 C) of 7 0 0 1 free from DNase and RNase contamination
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